Fig. 1

High-fat diet promotes post-traumatic osteoarthritis in mice. A Overview of the experimental design. Mice were fed either a low-fat diet (LFD) or a high-fat diet (HFD) for 12 weeks and sham or destabilization of the medial meniscus (DMM) surgery was performed at weeks 16–17. Sham group (n = 6), DMM group (n = 5). B Changes in weight over time. Arrow represents the time of the surgery. HFD increases body weight compared to LFD. C Representative histopathological section from mouse knee joints stained with fast green and Safranin-O. D Glasson Modified OARSI pathology score measuring osteoarthritic damage (n = 6 in the sham group, n = 5 in the DMM group). E Synovial thickness score (n = 6 in the sham group, n = 5 in the DMM group). Scale bar = 100μM. **P < 0.01, ****P < 0.0001. Two-way ANOVA Tukey’s multiple comparison test