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Fig. 2 | Arthritis Research & Therapy

Fig. 2

From: Expansion of myeloid-derived suppressor cells contributes to metabolic osteoarthritis through subchondral bone remodeling

Fig. 2

High-fat diet increases monocytic-MDSC expansion during PTOA. A Gating strategy used to define the M-MDSC subpopulation (CD11b+Ly6C+Ly6G− cells) in mouse bone marrow. Following the initial FS/SC discrimination, the gate was set on CD11b+ cells. After exclusion of doublets (not shown), live CD11b+ cells were gated and Ly6C+ and Ly6G+ populations. B Cytospin images of M-MDSC cells from the bone marrow from LFD and HFD mice (at 16 weeks). Scale bar denotes 10 microns. C HFD increases the BM M-MDSC population in mice (n = 4 in each group) by total number and percentage with concomitant suppression of CD8+ and CD4+ T-cells compared to LFD mice (n = 6 in each group, at 16 weeks). D HFD increases the BM M-MDSC population in DMM mice (n = 3 in each group). *P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001 by two-way ANOVA Tukey’s multiple comparison test

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