Fig. 1

rs17268364 risk G allele binds with EWSR1 and reduced gene expression. A In vitro luciferase assays in HEK293T cells showing reduced activity in risk allele constructs containing rs17268364 G allele (p = 0.002). B Protein mass spectrometry assay and Venn diagram with revealing the proteins pulled down by differentially binding with rs17268364 A or G allele from the lysates of HEK293 T cells. C An allele-specific band was observed in probes containing rs17268364 risk G allele. A supershift bind was detected with the rs17268364 risk G allele in addition of an anti-EWSR1 antibody. “+” and “−” mean added and unadded, respectively. Two independent experiments were performed with similar results