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Fig. 2 | Arthritis Research & Therapy

Fig. 2

From: Endothelial CCR6 expression due to FLI1 deficiency contributes to vasculopathy associated with systemic sclerosis

Fig. 2

The contribution of Fli1 deficiency to the up-regulated expression of CCR6 in endothelial cells. A mRNA levels of FLI1, CCL20, and CCR6 in human dermal microvascular endothelial cells (HDMECs) transfected with FLI1 siRNA or non-silencing scrambled RNA (SCR) were examined by qRT-PCR (n = 6 for each group). Results are expressed as means ± SEM. AU, arbitrary unit. B Protein levels of CCR6 and FLI1 in HDMECs transfected with FLI1 siRNA or SCR were examined by immunoblotting (n = 4 for each group). C Chromatin was isolated from HDMECs and immunoprecipitated using rabbit anti-FLI1 antibody or rabbit IgG. After isolation of bound DNA, PCR amplification was carried out using two sets of primers specific for the promotes of CCR6 transcript variant 1 (NM_004367) and CCR6 transcript variant 2 (NM_031409). D mRNA levels of CCR6 were determined by qRT-PCR in HDMECs treated with IL-17A (20 ng/mL), IL-10 (20 ng/mL), or TGF-β1 (2 ng/mL)

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