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Fig. 4 | Arthritis Research & Therapy

Fig. 4

From: Endothelial CCR6 expression due to FLI1 deficiency contributes to vasculopathy associated with systemic sclerosis

Fig. 4

CCR6 siRNA suppresses tubulogenic activity of HDMECs and in vivo neovascularization. A Tube formation assay was performed by applying HDMECs treated with Ccr6 siRNA or scrambled non-silencing RNA (SCR) onto the Matrigel and incubating for 24 h. To eliminate the influence of proliferation, cells were treated with mitomycin C before the assay. Representative images are shown (n = 5 for each group). The numbers of meshes, tubes, and intersections were counted manually. CCR6 mRNA levels were determined by qRT-PCR. B The number of dermal microvessels in the skin of BLM-treated mice subjected to Ccr6 siRNA or SCR was counted manually in the specimen subjected to hematoxylin and eosin staining (n = 6 for each group). Each graph represents mean ± SEM of the indicated parameters

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