Fig. 4

GSDMD deficiency attenuated articular cartilage degeneration and synovitis. Twelve-week-old Gsdmd^+/+ and Gsdmd^−/− male mice were subjected to sham or MLI surgery. A Representative images of Safranin-O staining of the left (sham) and right (MLI) knee joints. B Representative images of anterior synovium of sham or MLI knee joints from Gsdmd^+/+ and Gsdmd^−/− mice. C OARSI and synovitis scores. OARSI scoring was performed to quantify the severity of OA. Synovitis scores are based on the severity of synovial hyperplasia and sub-synovial inflammation. Reference data for C are shown in Fig. S3A. Data are mean ± SD. N = 7–8/group. Unpaired t test; *p < 0.05; **p < 0.01. Scale bar = 250 μm