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Fig. 3 | Arthritis Research & Therapy

Fig. 3

From: Effects of adenovirus-mediated knockdown of IRAK4 on synovitis in the osteoarthritis rabbit model

Fig. 3

Ad-shIRAK4 reduced the expression of IRAK4 and suppressed TLR/IL-1R signaling in the synovium from the osteoarthritis rabbit model. A IL-1β, IRAK4, TRAF6, IKK, and NFκB mRNA expression in synovial tissue was detected by qRT-PCR. B IRAK4, TRAF6, TAK1, p-IKK, p-NFκB, p38, and p-p38 protein expression in synovial tissue was detected by an automated western blot. Quantification of the relative protein expression via Image-J software, β-actin was served as the internal control. C Immunohistochemistry to identify IRAK4, TRAF6, and TAK1 in synovial tissue. D Positive staining for IRAK4, TRAF6, and TAK1were quantified by ImageJ software. Data are expressed as the mean ± SD. Statistical analysis was performed by one-way ANOVA, #P < 0.05 vs. control group; *P <0.05, **P <0.01, ***P <0.0001 vs. OA model group. n=3 per group. OA, OA model group; Ad-C, adenovirus empty vector group; Ad-shIRAK4-L, adenovirus low titer group; Ad-shIRAK4-H, adenovirus high titer group

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