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Fig. 5 | Arthritis Research & Therapy

Fig. 5

From: Hyaluronidase treatment of synovial fluid is required for accurate detection of inflammatory cells and soluble mediators

Fig. 5

Smaller immune cells remain in SF after centrifugation without hyaluronidase. A–C Treatment of SF and the analysis of cells were performed as described in Fig. 4, except for neutrophils, which were identified based on FSC/SSC. CD44 expression on CD4+ and CD8+ T cells, CD19+ B cells, and neutrophils. Flowcytometry plot of one representative donor is shown together with the summary of all patients. Each line represents a patient. n=7 patients. Wilcoxon signed-rank test was performed. D MFI values FCS-A of live cells determined by flowcytometry as described in Fig. 4. Each dot represents one patient. n= 12 patients. Mean and 95% shown in lower panel. Wilcoxon signed-rank test was performed

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