Fig. 3

The integrating analysis of individual-level DNA methylation and mRNA expression data. A Venn diagram depicting differentially methylated positions related genes (DMGs) and differentially expressed genes (DEGs) between AS cases and health controls (HCs). B Starburst plot of gene expression changes (log2 FC) vs. DNA methylation changes (Δβ). Red dots represented the probes with significantly differential methylation and differential expression. FC, fold change. C Top 20 KEGG biological pathways of 112 DEGs whose expression levels are correlated with the corresponding methylation levels. The orange squares denote that gene present in the pathway. D The protein-protein interactions network among the correlated genes with STRING software. The sizes of the nodes are correlated with the degree (the connected number). The blue, orange, and the red denote the degree from 1 to 4, from 5 to 9, above 10, respectively