Fig. 1

ECV304 produces high levels of citrullinated proteins. A WB of citrullinated proteins detected by RA serum in different cells lines. Total cell lysates were incubated under citrullination conditions followed by SDS-Page fractionation. Bands were revealed using an ACPA-positive RA serum and an anti-human IgG, HRP-conjugate. B Although T24 and ECV304 cell lines derive from the same individual, they differ in their citrulline content (n = 3, p < 0.05, unpaired T test). C Agarose gel electrophoresis depicting RT-PCR products for amplification of PAD enzyme isotype mRNA. ECV304 has higher mRNA levels of PAD2 and PAD3 isotypes