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Fig. 2 | Arthritis Research & Therapy

Fig. 2

From: Acazicolcept (ALPN-101), a dual ICOS/CD28 antagonist, demonstrates efficacy in systemic sclerosis preclinical mouse models

Fig. 2

Acazicolcept (ALPN-101) alleviates dermal fibrosis development in the HOCL mouse model. A Mean percentage of body weight change calculated between first week and sixth week of treatment. B Measurements of skin fold thickness in millimeters from week 1 to week 6, collected weekly. C Left: Representative Sirius Red 4 μm-skin sections of PBS-, HOCL/Fc control-, and HOCL/acazicolcept-treated mice (objective × 8). Arrows represents the dermal thickness measurement on each skin section. Right: Mean of five measurements of dermal thickness for each mouse in micrometers. D Content of collagen in a 3-mm dorsal skin punch evaluated by Sircol assay in PBS-, HOCL/Fc control-, and HOCL/acazicolcept-treated mice. E Left : Representative SHG images of 16 μm dorsal skin sections from PBS-, HOCL/Fc control-, and HOCL/acazicolcept-treated mice. Right : Scoring of fibrillar collagen in PBS-, HOCL/Fc control-, and HOCL/acazicolcept-treated dorsal skin sections. F Left: Representative IHC staining of alpha-SMA (brown) in 4-μm skin sections counterstained with hematoxylin of PBS-, HOCL/Fc control-, and HOCL/acazicolcept-treated mice (magnification × 8 and × 100). Right: Number of alpha-SMA positive cells in a 4-μm dorsal skin section from PBS-, HOCL/Fc control-, and HOCL/acazicolcept-treated mice. Mice were divided into three groups: PBS/treated group (n = 6), HOCL/Fc control-treated group (n = 8), and HOCL/acazicolcept-treated group (n = 8). Horizontal lines represent the median with range. *p < 0.05 ; **p < 0.01 ; ***p < 0.001 by Mann-Whitney U test

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