Fig. 3

Acazicolcept (ALPN-101) decreased immune cell infiltrates in lesional skin of HOCL-treated mice. A Representative IHC staining (brown) of macrophages (CD68+), neutrophils (Ly6G+), B cells (CD20+), and T cells (CD3+) on 4-μm dorsal skin sections counterstained with hematoxylin from PBS-, HOCL/Fc control-, and HOCL/acazicolcept-treated mice (magnification left × 6 right : × 120). B Number of CD68, Ly6G, CD20, or CD3-positive cells in a 4-μm dorsal skin section from PBS-, HOCL/Fc control-, and HOCL/acazicolcept-treated mice. Mice were divided into three groups: PBS group (n = 6), HOCL/Fc control-treated group (n = 8), and HOCL/acazicolcept-treated groups (n = 8). Horizontal lines represent the median with range. *p < 0.05; **p < 0.01 ; ***p < 0.001 by Mann-Whitney U test. ns = not significant