Single-cell transcriptomics of popliteal lymphatic vessels and peripheral veins reveals altered lymphatic muscle and immune cell populations in the TNF-Tg arthritis model

Table 1 Changes in cell clusters from the peripheral vasculature between WT and TNF-Tg mice by scRNAseq. The cluster numbers and cell types are aligned with those defined in Fig. 3. The cell numbers associated with each cluster are provided for both wild-type (WT) and tumor necrosis factor transgenic (TNF-Tg) groups along with the total cell counts for each cluster when these datasets are integrated together. To examine the changes in cell populations between the WT and TNF-Tg conditions, a percentage for each cell type was calculated by the number of cells in an individual cluster divided by the total cells within a single group (WT or TNF-Tg)

Cluster	Cell Type	WT #	WT %	TNF #	TNF %	Total #
1	LMCs	1433	26.11	552	16.28	1985
2	M2-Macrophages 1	1111	20.24	269	7.93	1380
3	VSMCs	751	13.68	580	17.10	1331
4	M1-Macrophages	522	9.51	331	9.76	853
5	Neutrophils	344	6.27	228	6.72	572
6	Inf Monocytes	66	1.20	494	14.57	560
7	Dendritic Cells 1	152	2.77	261	7.70	413
8	Dendritic Cells 2	306	5.58	24	0.71	330
9	Dying Cells	236	4.30	67	1.98	303
10	Pat Monocytes	7	0.13	192	5.66	199
11	Myeloid Progenitors	74	1.35	49	1.45	123
12	Mast Cells	98	1.79	17	0.50	115
13	Prolif Monocytes	70	1.28	45	1.33	115
14	T-Cells 1	45	0.82	64	1.89	109
15	Fibroblasts	75	1.37	30	0.88	105
16	RBCs	54	0.98	46	1.36	100
17	M2-Macrophages 2	80	1.46	14	0.41	94
18	Basophils	7	0.13	64	1.89	71
19	T-Cells 2	31	0.56	34	1.00	65
20	Endothelial Cells	26	0.47	30	0.88	56
	Total	5488	100	3391	100	8879

ISSN: 1478-6362