Fig. 4

Adipogenic potential of SF subsets. A Oil-red staining. The SF subsets cultured in an adipocyte differentiation medium for 3 weeks. Adipogenesis was detected as red spots with oil-red staining. B qPCR of adipogenic differentiation markers. The relative expression levels of LPL and PPARγ in differentiated cells. Cells per donor were cultured in 3 dishes and the results of 7 donors. The target genes were normalized with 18S of undifferentiated SF as a control. OA and RA samples are plotted as white circles and white squares, respectively. The average data of each subset are plotted as black squares. Data were analyzed by Dunn’s multiple comparison test for comparing each subset from the same samples