Fig. 4

The effect of oral LG283 on the expression of cytokines and Smad3. Crude lysate and mRNA were extracted from skin samples of placebo- and LG283-treated mice on day 7 after bleomycin injection. a Concentration of the indicated cytokines was examined by cytometric bead array. Values represent mean ± SEM; n=5 each group; **p< 0.01. b αSMA expressing cells were quantified by immunohistochemistry. Representative images are shown (left). Scale bar, 50 μm. Quantitative analysis is shown in the bar graph (right). Values represent mean ± SEM; n =3 each group. **p< 0.01. c, d mRNA and protein concentration of TGF-β1 were evaluated with real-time RT-PCR of the skin and enzyme-linked immunosorbent assay of the skin extracts, respectively. Values represent mean ± SEM; n = 5 each group. e Protein expression levels of Smad3 and p-Smad3 were quantified using western blotting. Values were normalized to tubulin levels and were shown as the mean of fold change compared to vehicle control; n=3 each group. Values represent mean ± SEM; n = 5 each group; **p< 0.01. Molecular weights of the paneled proteins; 53 kDa (Smad3 and pSmad3) and 50 kDa (TUBULIN)