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Fig. 4 | Arthritis Research & Therapy

Fig. 4

From: Effects of local cryotherapy on systemic endothelial activation, dysfunction, and vascular inflammation in adjuvant-induced arthritis (AIA) rats

Fig. 4

Leucocyte infiltration in aorta from AIA rats and effect of cryotherapy. AH Flow cytometry analyses of CD45+ populations on rat thoracic aorta from control and AIA rats at the acute inflammatory phase (day 24 post-immunization). IP Effect of local cryotherapy in AIA rats treated from day 11 to day 24 post-immunization. Different leucocyte subpopulations were studied: monocytes/macrophages CD11b/c+ RP-1 (B, J), neutrophils CD11b/c+ RP-1+ (C, K), T lymphocytes CD3+ (D, L), CD4+ (E, M), CD8+ (G, O), CD4+ IL-17A+ (F, N) and CD8+ IL-17A+ (H, P) T cells. Gating strategies are shown in Supplementary Fig. 1A–J. Data are presented as the number of stained cells per mg of the aorta. Results are expressed as means ± SEM (n=10 rats/group). *(p<0.05), **(p<0.01), ***(p<0.001). NS, non-significant

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