Fig. 6

Circulating leucocytes in AIA rats and effect of cryotherapy. A–G Effect of AIA at the acute inflammatory phase (day 24 post-immunization) on leucocytes populations in the blood as compared to controls. H–N Effect of local cryotherapy on blood leucocytes and leucocyte subpopulations in AIA rats. Flow cytometry analysis assessed the absolute number of blood leucocytes (CD45⁺ cells, A, H) and of the different leucocyte subpopulations: CD3⁺ T lymphocytes (B, I), CD11b/c⁺ monocytes/macrophages (C, J), CD4⁺ (D, K), CD8⁺ (F, M) T cells, and intercellular IL-17A⁺ CD4⁺ (E, L) or CD8⁺ (G, N) T cells. Gating strategies are shown in Supplementary Fig. 1K-R. Data are expressed as number of stained cells per μl of blood. Results are expressed as means ± SEM (n=10 rats/group). *(p<0.05), **(p<0.01), ***(p<0.001). NS, non-significant