Fig. 3From: The anterior cruciate ligament in murine post-traumatic osteoarthritis: markers and mechanicsRepresentative histological staining in the anterior cruciate ligament (ACL) of control and post-trauma mouse knee joints. A, B Toluidine blue (Tol. Blue) staining of healthy and post-trauma ACL showed Tol. Blue staining in the mid-substance and tibial enthesis of the post-trauma ACL (black arrow). C, D Brightfield Picrosirius Red (BF Pic. Red) staining confirmed rounded-cell morphology in the tibial enthesis of the post-trauma ACL (black arrow). E, F Polarised Picrosirius Red (Pol Pic. Red) showed red collagen birefringence in the tibial enthesis extending towards the mid-substance of the post-trauma ACL (blue arrows). Scale is 100 μm and 50 μm for lower and higher magnification. Post-trauma ACL images are from + 4-weeks post-traumaBack to article page