Fig. 2

Principal component analysis (PCA)-pairwise differential expression. Volcano and box plots of statistically differently regulated proteins (for the volcano plot, red when osteoarthritis (OA) was lower than controls and blue when OA was higher than controls between A OA-obese (n=10, OA-ob. [hatched]) and controls (n=8, CTL [white]), B OA-non obese (n=10, OA-non ob. [hatched]) and controls (n=8, CTL [white]) and C OA-obese (n=10) and OA-non obese (n=10)). The intensity values for each protein and sample were obtained from the mass spectrometry analysis (refer to Table S2) and transformed as Log₂. Statistical analysis used the Limma method, and q<0.050 was considered statistically significant; a strong trend toward statistical difference is indicated in italic. ACT, actins; ADIPOQ, adiponectin; CRP, C-reactive protein; CRTAC1, cartilage acidic protein 1; FBN, fibrillin 1; IGHV3-35, Ig heavy variable 3-35; KDM4C/4B/4E, lysine-specific demethylase4C/4E/4B; KHSRP, KH-Type Splicing Regulatory Protein; LYZ, lysozyme; PTGDS, prostaglandin-H2 D-isomerase; S100A9: S100 Calcium Binding Protein A9