Table 1 Primers for real-time PCR
Target gene | Primers pairs |
|---|---|
β-actin | Forward 5’-CCTGTACGCCAACACAGTGC-3’ Reverse 5’-ATACTCCTGCTTGCTGATCC-3’ |
Piezo1 | Forward 5’- GAGATGATGGACAGAGAC-3’ Reverse 5’-AGTAATGGCTAAGGAAGAC -3’ |
p53 | Forward 5’-AGAGCTGAATGAGGCCTTGGAA-3’ Reverse 5’-GAGTCAGGCCCTTCTGTCTTGAAC-3’ |
p16 | Forward 5’-CGGTCGTACCCCGATTCAG-3’ Reverse 5’- GCACCGTAGTTGAGCAGAAGAG-3’ |