Fig. 1

The enhanced expression of Sema3G in the inflamed synovium in humans and mice. A Genes differentially expressed before and after MTX treatment. Genes differentially expressed are highlighted in red (upregulated after MTX) or blue (downregulated after MTX). B Sema3G expression in the synovium of OA or RA patients. The synovium specimens were stained with anti-Sema3G antibody and visualized with DAB. Bars indicate 200 μm (Low magnification) or 20 μm (high magnification). C The cumulative data of the Sema3G-positive area. Data are expressed as the means ± SEM. The statistical analyses were performed using an unpaired t-test. D Sema3G expression in the synovium of CIA. The hind paws of the control and CIA-induced mice were stained with anti-Sema3G antibody. The representative data are shown. Similar results were obtained in three independent experiments. Bars indicate 200 μm