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Fig. 1 | Arthritis Research & Therapy

Fig. 1

From: Myeloid cell-specific deletion of Capns1 prevents macrophage polarization toward the M1 phenotype and reduces interstitial lung disease in the bleomycin model of systemic sclerosis

Fig. 1

Calpain activity increases in bleomycin model of SSc-ILD and Characterization of mice with LYZ-specific Capns1 knockout. A Calpain activity was measured in lysates of lung tissue from sham treated mice (Control) or mice treated with BLM. Data was compared by Student t-test. B Representative RT-PCR for GAPDH and Capns1 mRNA from six mice in each group. Levels of Capns1 mRNA were significantly reduced in Capns1-ko macrophages. In contrast, the Capns1-ko mRNA was not decreased in Capns1-ko lymphocytes. C Capns1-ko mRNA was detected by RT-PCR in wild-type and Capns1-ko macrophages and lymphocytes and normalized with GAPDH mRNA. The test of homogeneity of variance showed P > 0.1, ANOVA test was applied. D Representative western blots for CAPN1 and CAPN2 proteins from macrophages (left) and lymphocytes (right). E Both CAPN1 and CAPN2 protein levels were significantly reduced in Capns1-ko macrophage but not in lymphocytes. The test of homogeneity of variance showed P < 0.1, nonparametric test was applied. Data are mean ± SD. n = 6–10; *: P < 0.05, vs. sham group in WT mice; #: P < 0.05, vs. bleomycin model of SSc group in WT mice. OD optical density

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Arthritis Research & Therapy

ISSN: 1478-6362

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