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Fig. 2 | Arthritis Research & Therapy

Fig. 2

From: Myeloid cell-specific deletion of Capns1 prevents macrophage polarization toward the M1 phenotype and reduces interstitial lung disease in the bleomycin model of systemic sclerosis

Fig. 2

Histological analyses of inflammation and fibrosis in SSc skin tissues. A Skin sections of sham treated (Control) or BLM treated (bleomycin model of SSc) WT or Capns1-ko mice were stained with hematoxylin and eosin (H&E, upper, Scale bars, 50 μm, magnification X200) or Masson’s trichrome (lower, Scale bars, 100 μm, magnification X200). B Dermal thickness (µM) was quantified by histopathologic analysis of H&E stained sections. The test of homogeneity of variance showed P > 0.1, ANOVA test was applied. C Collagen deposition was quantified as the % Masson’s trichrome stained mean area. The test of homogeneity of variance showed P < 0.1, nonparametric test was applied. D The hydroxyproline (HYP) content in skin tissues was determined biochemically and expressed as µg HYP per gram of wet tissue. The test of homogeneity of variance showed P > 0.1, ANOVA test was applied. Data are mean ± SD. n = 8, *: P < 0.05, vs. Control WT mice; #: P < 0.05, vs. bleomycin model of SSc WT mice. OD optical density

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Arthritis Research & Therapy

ISSN: 1478-6362

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