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Fig. 2 | Arthritis Research & Therapy

Fig. 2

From: Modulation of B cell activation by extracellular vesicles and potential alteration of this pathway in patients with rheumatoid arthritis

Fig. 2

Low frequency of CD69+ and CD86+ cells and proliferation of in vitro-activated B cells exposed to m/lEVs. A Representative contour plots of CD69 expression in B cells from HD cultured for 24 h without stimulation (unstimulated, Unst) or with anti-BCR in the absence or presence of m/lEVs from HD (HD-m/lEVs), patients with RA (PRA-m/lEVs), and patients with RA-forming immune complexes (PRA-m/lEV-ICs). B Frequency of CD69+ (left panel), CD80+ (center panel), and CD86+ (right panel) B cells, cultured for 24 h without stimulation or with anti-BCR in the absence or presence of HD-m/lEVs, PRA-m/lEVs, or PRA-m/lEV-ICs. The data of five HD donors and medians are shown. C The frequency of CD69+ (left panel), CD80+ (center panel), and CD86.+ (right panel) B cells, cultured for 72 h without stimulation or with anti-BCR and CpG in the absence or presence of HD-m/lEVs, PRA-m/lEVs, or PRA-m/lEV-ICs. The data of eight HD donors and medians are shown. D Representative histogram of CellTracer expression on B cells cultured for 72 h (left panel), consolidated data of proliferating B cells (center panel), and the index of division (right panel) of B cells cultured for 72 h without stimulation or with anti-BCR + CpG in the absence or presence of HD-m/lEVs, PRA-m/lEVs, or PRA-m/lEV-ICs. Data of 6 − 8 HD donors and median are shown. B–D Kruskal − Wallis test with Dunn’s post-test. * p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001

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