Fig. 6

The phosphorylation levels of signaling proteins are increased after a single bout of HIIT. Representative western blots of the total and phosphorylated AMPK Thr172, CaMKII Thr286 (A), ACC Ser79, and p38 MAPK Thr180/Tyr182 (D) of the gastrocnemius muscles in control (CNT) and EAM mice with or without one bout of high-intensity interval training (HIIT; Exp 2). Bars showing the mean and SEM of the phosphorylation levels of AMPK Thr172 (B), CaMKII Thr286 (C), ACC Ser79 (E), and p38 MAPK Thr180/Tyr182 (F) relative to total protein content. Data expressed relative to the mean in the CNT muscles, which was set to 100%; n = 10 muscles per group. One-way ANOVA with Tukey post hoc test was performed, except for the phosphorylation level of ACC where a Kruskal-Wallis one-way ANOVA was used on ranks. *P < 0.05 vs CNT, ^#P < 0.05 vs EAM