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Fig. 4 | Arthritis Research & Therapy

Fig. 4

From: Increased Wnt/β-catenin signaling contributes to autophagy inhibition resulting from a dietary magnesium deficiency in injury-induced osteoarthritis

Fig. 4

Activation of Wnt/β-catenin signaling contributed to reduction in autophagy. A Mouse chondrocytes were cultured under different magnesium conditions with or without XAV939 pretreatment. Protein levels of LC3-II and Beclin-1 under different magnesium conditions were analyzed by western blot. B The quantification of protein expression of LC3-II and Beclin-1 was done by densitometry analysis of the protein bands. Values were normalized to β-tubulin (n = 5). C Chondrocyte imaging with DALGreen staining. Autolysosomes were marked with white arrows. Scale bar: 5 μm. D Number of autolysosomes per cell was quantified. Data were expressed as the mean ± SD and analyzed by one-way ANOVA test. (*P< 0.05, **P< 0.01, ***P< 0.001, ****P< 0.0001)

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Arthritis Research & Therapy

ISSN: 1478-6362

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