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Fig. 5 | Arthritis Research & Therapy

Fig. 5

From: Increased Wnt/β-catenin signaling contributes to autophagy inhibition resulting from a dietary magnesium deficiency in injury-induced osteoarthritis

Fig. 5

Impaired magnesium homeostasis induced by magnesium deficiency was mediated by TRPM7. A Representative fluorescence images showing the concentration of intracellular Mg2+ of chondrocytes cultured under different magnesium conditions (0.7 mM, 0.4 mM, and 0.1 mM) for 48 h. B Intracellular Mg2+ concentration quantified by measuring the intensity of fluorescence (n = 10). C Protein level of TRPM7 under different magnesium conditions for 48 h were analyzed by western blot. D The quantification of protein expression of TRPM7 was done by densitometry analysis of the protein bands. Values were normalized to β-tubulin (n = 3). E, F Immunostaining and quantitative analysis of cells positive for TRPM7 in sham and DMM-induced OA mice fed with diets containing different amounts of magnesium (500 mg/kg, 300 mg/kg, 100 mg/kg) (n = 9). Scale bar: 100 μm. Data were expressed as the mean ± SD and analyzed by one-way ANOVA test (*P< 0.05, **P< 0.01, ***P< 0.001, ****P< 0.0001). G Summary of the present study: Extracellular magnesium deficiency downregulates the expression of TRPM7, resulting in reduced intracellular magnesium. Subsequently, intracellular magnesium deficiency inhibits autophagy of chondrocytes by the activation of the Wnt/β-catenin signaling pathway and thus aggravates cartilage damage

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