Fig. 2

CC-99677 inhibited the production of TNF, IL-6, IL-17A, and MCP-1 from SEB-1/IL-2-stimulated human PBMCs. TNF (a), IL-6 (b), IL-17A (c), and MCP-1 (d) inhibition in SEB/IL-2-stimulated PBMCs from patients with AS and healthy volunteers. PBMCs were treated with CC-99677 for 1 h followed by SEB + IL-2 stimulation for 72 h. Magnetic beads were used to assess TNF, IL-6, IL-17A, and MCP-1. Each sample was analyzed in duplicate, and data were normalized to the stimulation control (100%). The mean percent inhibition is indicated by a horizontal line with error bars for the standard error of the mean; significance was calculated using the one-way ANOVA with Dunnett’s post-test algorithms in GraphPad Prism version 5.01. Gray symbols indicate the data points from healthy donors (n = 10), and blue symbols indicate the data points from donors with AS (n = 30). ANOVA, analysis of variance; AS, ankylosing spondylitis; IL, interleukin; MCP-1, monocyte chemoattractant protein 1; PBMC, peripheral blood mononuclear cell; SEB, Staphylococcal enterotoxin B; stim ctrl, stimulation control; TNF, tumor necrosis factor. ****p < 0.0001; ***p < 0.001; **p < 0.01; *p < 0.05