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Fig. 4 | Arthritis Research & Therapy

Fig. 4

From: Targeting clock-controlled gene Nrf2 ameliorates inflammation-induced intervertebral disc degeneration

Fig. 4

SFN attenuated si-Bmal1-induced the dysfunction of NP cells. The NP cells were divided into four groups, with no treatment, incubated with 5 μM SFN, si-Bmal1, and si-Bmal1 + 5 μM SFN. a Western blotting of BMAL1, NRF2, Aggrecan, and MMP13 protein expression (normalized to GAPDH expression). b Immunofluorescence staining of NRF2, Aggrecan, and MMP13 staining in Control, SFN, si-Bmal1, and si-Bmal1 + 5 μM SFN group (scale bar: 25 μm). c The levels of TNF-α and IL-1β protein. n = 3, NS, not significant difference, *P<0.05, **P < 0.01. d, e DCFDA staining images and quantification (scale bar: 50 μm). f, g TUNEL staining images and quantification (scale bar: 25 μm)

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