Fig. 2
VGLL3-regulated genes in RA-FLS. a RNA sequencing (RNA-seq) of RA-FLS transfected with Flag-VGLL3 expressing lentivirus or the vector. The expression levels of the VGLL3-regulated genes were shown as log2(FC) in the heatmap. N = 1. b Gene set enrichment analysis (GSEA) was performed using the RNA-seq data and the top 10 enriched gene sets were demonstrated. c The expression levels of the IFN-stimulated genes after VGLL3 overexpression were shown as log2(FC) in the heatmap. d, e The mRNA expression levels of STAT1, TNFSF13B, MX1, IRF7, OAS1, TLR3, and CCL5 were examined by qPCR upon VGLL3 overexpression (d) or VGLL3 knockdown (e). N = 3. f, g The protein expression levels of VGLL3, STAT1, and MX1 were detected by Western blotting upon VGLL3 overexpression (f) or knockdown (g). GAPDH was used as the housekeeping gene. Representative images of at least three independent experiments were shown in the Western blotting experiments. * p < 0.05, ** p < 0.01, *** p < 0.001. Scr siRNA: scrambled siRNA. Data were shown as mean ±SD