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Fig. 7 | Arthritis Research & Therapy

Fig. 7

From: Gingko biloba-inspired lactone prevents osteoarthritis by activating the AMPK-SIRT1 signaling pathway

Fig. 7

The anti-arthritic effects of bilobalide were abolished when AMPK was inhibited. A Immunofluorescence staining showed that Compound C treatment reduced phospho (P)-AMPKα expression levels. B Immunofluorescence staining showed SIRT1 expression levels that were inhibited. C Quantitative analysis of P-AMPKα and SIRT1 activity. D SIRT1 activity was inhibited by the treatment with Compound C. E Using real-time reverse-transcription-polymerase chain reaction and GAPDH as an internal reference, the transcript levels of Col2a1 and Acan were determined. F, G Western blot assays were used to determine the protein levels of P-AMPKα, AMPKα, SIRT1, COLII, ACAN, MMP13, and ADAMTS5. The values represent the mean ± standard deviation. Each in vitro experiment was repeated three times or more. Statistically, significant differences between the indicated groups are indicated by *p < 0.05, **p < 0.01, ***p < 0.001, or ****p < 0.0001

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