Fig. 8

Bilobalide activated AMPK-SIRT1 protected articular cartilage extracellular matrix in vivo. A, B Representative images of a COLII-stained plot with percentages of COLII-positive cells were quantified. C, D Representative images of MMP13-stained plot and quantification of the percentage of MMP13-positive cells. E, F Representative images of phospho (P)-AMPKα-stained plot and quantification of the percentage of P-AMPKα-positive cells. G, H Representative images of SIRT1-stained plot and quantification of the percentage of SIRT1-positive cells. Each in vivo experiment was repeated six times or more. Statistically, significant differences between the indicated groups are indicated by ^*p < 0.05, ^**p < 0.01, ^***p < 0.001, or ^****p < 0.0001