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Fig. 3 | Arthritis Research & Therapy

Fig. 3

From: Circ0007042 alleviates intervertebral disc degeneration by adsorbing miR-369 to upregulate BMP2 and activate the PI3K/AKt pathway

Fig. 3

circ_7042 regulates miR-369-3p/CDH2. A, B The expression levels of the miR-369-3p and CDH2 genes were detected by qPCR. C The localization and expression level of CDH2 protein in NP were detected by immunofluorescence. D Targeted binding site of circ_7042 to miR-369-3p and mutation sequence of circ_7042. E Dual-luciferase activity; the fluorescence detection results were statistically quantified. F After miR-369 was adsorbed by RNA pull-down experiments, the circ_7042 gene was amplified by PCR, and agarose gel electrophoresis results were obtained. G The relative expression level of the circ_7042 gene in RNA enriched by the RNA pull-down assay was detected by qPCR. *P<0.05, ** P<0.01, the two groups were compared between the wires

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