Fig. 5

miR-369-3p regulates NP cell apoptosis through BMP2. A Targeted binding sequence and mutation sequence of miR-369-3p and BMP2-3′UTR. B Dual-luciferase activity and the statistical quantification results of fluorescence intensity. C After the RNA pull-down experiment, the RNA adsorbed by miR-369 was amplified by PCR, and agarose gel electrophoresis revealed the amplification band of the BMP2 gene. D The relative expression level of the BMP2 gene in the RNA pull-down experiment was detected by qPCR. E The relative expression level of the BMP2 gene after transfection with miR-369 mimic or inhibitor was detected by qPCR. F Western blotting was used to detect the relative expression level of BMP2 protein after transfection with miR-369 mimic or inhibitor. G Statistical quantification results of the optical density values of the immunoblotting bands. H The inhibitory effects of different siRNAs on the BMP2 gene were detected by qPCR. I qPCR verified the regulation of BMP2 gene expression levels by si-BMP2, circ-7042, and miR-369 inhibitor. J Apoptosis levels were measured by flow cytometry with the Annexin V-FITC fluorescence intensity on the horizontal axis and the PI staining fluorescence intensity on the vertical axis. R2 represents necrotic cells, R3 represents late apoptotic cells, R4 represents normal cells, and R5 represents early apoptotic cells. K The apoptosis level was detected by flow cytometry, and the percentage of apoptotic cells was statistically quantified. *P<0.05, **P<0.01, the two groups were compared between the wires