Fig. 3

Microarray-based gene expression profiling of lung tissue in the BLM-induced SSc model treated with MT-7117. Lung samples treated with MT-7117 at 0.3 mg/kg from the BLM-induced SSc model (prophylactic model) were used for DNA microarray analysis. Bioinformatics analysis was performed using IPA software, and two types of calculations of two data sets were performed: “Canonical pathways” and “Diseases or functions.” Downstream effect analysis was used to calculate the activation z-score. A Principal component analysis of gene expression profiles in PBS_vehicle vs. BLM_vehicle vs. BLM_MT-7117 groups. B Categories by cell types that changed with BLM and MT-7117 treatment. Categories (C) and signaling pathways (D) related to SSc pathophysiology that changed with BLM and MT-7117 treatment. The color of symbols represents activation (red) or inactivation (green), and the size reflects the p-value. E Fluctuating genes involved in IL-6 signaling. F Pathway map of IL-6 signaling: fluctuations in PBS vs. BLM groups (left) and BLM vs. MT-7117 groups (right). Red: increased expression; green: decreased expression; solid line: direct relation; dotted line: indirect relation