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Fig. 3 | Arthritis Research & Therapy

Fig. 3

From: Factors secreted by monosodium urate crystal-stimulated macrophages promote a proinflammatory state in osteoblasts: a potential indirect mechanism of bone erosion in gout

Fig. 3

Effects of conditioned medium from MSU crystal-stimulated RAW264.7 macrophages on factor secretion from MC3T3-E1 cells. RAW264.7 macrophages were cultured with the indicated concentrations of MSU crystals for 24 h. Conditioned medium from the RAW264.7 cultures was added to MC3T3-E1 cells at 40% of the final volume. The graphs on the left present gene expression determined by real-time PCR; the graphs on the right present concentrations of secreted factors determined by ELISA. A Gene expression and secretion of OPG. B Gene expression and secretion of IL-6. C Gene expression of Ptgs2, encoding the COX-2 enzyme, and secretion of PGE2. Means (SEM) of data pooled from three or more biological repeats are presented. Data were analyzed by two-way ANOVA with post hoc Dunnett’s test. *p < 0.05, **p < 0.01, and ***p < 0.001 versus control conditioned medium at that time point; MSU CM, concentrations of MSU crystals used to prepare conditioned medium from RAW264.7 cells

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