Fig. 7
Effects of COX-2 inhibition on HOB response to conditioned medium from MSU crystal-stimulated THP-1 monocytes. Conditioned medium was collected from THP-1 monocytes cultured with or without 0.5 mg/mL MSU crystals for 20 h. The COX-2-selective inhibitor SC-236 was added to HOB cells for 1 h prior to the addition of conditioned medium at 40% of the final volume. A Gene expression analyzed by real-time PCR. B Secretion of PGE2 and IL-6 from HOBs. Data shown are pooled from three biological repeats and are presented as mean (SEM). Data were analyzed by two-way ANOVA with post hoc Sidak’s test. **p < 0.01 and ***p < 0.001 versus control conditioned medium at that time point. Cnt CM and MSU CM, conditioned medium from THP-1 cells incubated without MSU and with 0.5 mg/mL MSU, respectively