Fig. 4

IL-23 contribution to Th17/Th1 cytokine production. PBMCs were cultured in co-culture with synoviocytes or non-lesional (NLSF) or lesional skin fibroblasts (LSF) at a 5:1 ratio for 48 h, in the presence of PHA (5 μg/ml). IL-23 (50 ng/ml) or anti-IL-23 antibody (1 μg/ml) was added in culture (A). An antibody control was also used at 1 μg/ml (B). Production of IL-6, IL-1β, IL-23, IL-17, IL-12, and IFNγ in cell supernatants was measured by ELISA. “*” compares the condition of culture (IL-23 or anti-IL-23 vs. control). *p < 0.05, Wilcoxon test. n = 7 experiments in (A) and n = 3 in (B)