Fig. 2

Stimulation with TNFα increased TET3 expression levels in RA FLS. A Relative mRNA expression levels of TET1/2/3. RA FLS (n = 4). B Immunoblotting analysis of TET3 expression in RA FLS unstimulated or stimulated with TNFα for 0, 48, and 96 h. RA FLS (n = 3). C (Left) Representative patterns in immunohistochemical staining of TET3 expression in RA FLS stimulated with TNFα for 0, 24, and 48 h. (Right) The nucleus/cytoplasm (N/C) intensity ratio of TET3 expression (20 cells each). RA FLS (n = 3). D (Left) Immunoblotting with 5hmC in gDNA from FLS unstimulated for 0 h and 96 h or stimulated with TNFα for 96 h. (Right) Relative expression levels of 5hmC. FLS (n = 3). Data are mean ± standard error of the mean (A and D) and the box-and-whisker plots (C). Two-tailed P values by the Mann-Whitney U-test (vs. no-stimulation control), *P < 0.05 (A) and the Tukey’s honestly significant difference test (C). One-tailed P values by the Mann-Whitney U-test (D)