Fig. 3

Identification of TNFα-TET3-dependent genes, pathways, and RA-like phenotypes in RA FLS. A The Venn diagram of induced and downregulated genes. B Representative enriched KEGG pathways. C Heat map of chemokine genes expression. Red corresponds to gene upregulation and blue to gene down-regulation. D Relative mRNA expression levels in RA FLS with or without TNFα stimulation and TET3-knockdown. CXCL8 (n = 3), CCL2 (n = 3), RANKL (n = 3), OPG (n = 3), MMP1 (n = 3), MMP13 (n = 3). E Concentrations in the supernatant of FLS with or without TNFα stimulation and TET3-knockdown. RA FLS (n = 4). F (Left) Representative images of scratch assays of RA FLS with or without TNFα stimulation and TET3-knockdown. (Right) Count of cells per field filling the original scratch area. RA FLS (n = 3). Data are mean ± standard error of the mean (D, E, and F). One-tailed P values by the Mann-Whitney U-test (D, E, and F)