Fig. 4

The three most populated cell-type subpopulations across JIA subtypes were identified as chondrocyte-like, fibroblast-like, and smooth muscle cell-like cells. For oligoarticular JIA FLS samples, 38% of total cells were labeled as chondrocyte-like, 24.5% of total cells represented fibroblast-like cells, and 22.5% made up the smooth muscle cell-like cell subpopulation (A). 39% of total cells identify as chondrocyte-like, 28% of total cells are labeled as fibroblast-like, and 10% of total cells consisted of smooth muscle cell-like cells in the groups of ETB JIA FLS samples (A). Lastly, polyarticular JIA FLS had 71% of total cells that were chondrocyte-like, 12% of total cells were fibroblast-like, and 5% of total cells were labeled as smooth muscle cell-like cells (A). There was a − 8.89 fold-decrease of smooth muscle cell-like cells between oligoarticular JIA FLS samples and polyarticular JIA FLS samples (^p = 0.0001) (B). Fibroblast-like cell percentage was significantly higher in ETB JIA FLS than oligoarticular JIA FLS (*p = 0.0297) (B). Chondrocyte-like cell percentage significantly increased in polyarticular JIA FLS compared to oligoarticular JIA FLS (^p = 0.0006) and ETB JIA FLS (φp = 0.0044) (B)