Fig. 5
From: Senescence-accelerated mice prone 8 (SAMP8) in male as a spontaneous osteoarthritis model
Osteoarthritis related markers in the articular cartilage of SAMP8. A The expression pattern of type X Collagen in articular cartilage of SAMR1 and SAMP8 at 4 and 6 weeks of age. Knee joints were assessed by immunohistochemistry. B Medial knee joints from SAMR1 and SAMP8 at 6, 9, and 11 weeks of age (n = 6 for each strain) were assessed by TUNEL staining. White lines: outline of articular cartilage/meniscus; Dashed lines show borders between middle/deep zone or deep zone/subchondral bone. C Knee joints from SAMR1 and SAMP8 at 4, 6, 9, and 11 weeks of age (n = 5 for each strain) were assessed by immunohistochemistry using anti-p16INK4a antibody. D Immunohistochemistry using anti-ADAMTS-5 and anti-MMP-13 antibodies in medial compartment of the knee from SAMR1 and SAMP8 at 6, 9, and 11 weeks of age (n = 5 for each strain). All data are represented as mean ± S.D. Comparison of mean values was performed using Welch’s t test. The p-values (#P < 0.05, ##P < 0.01) were corrected using the Holm-Sidak method for multiple comparison correction