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Fig. 2 | Arthritis Research & Therapy

Fig. 2

From: Absence of Epstein-Barr virus DNA in anti-citrullinated protein antibody-expressing B cells of patients with rheumatoid arthritis

Fig. 2

BNRF1 is undetectable in PB antigen-specific ACPA-expressing B cells. B2M and BNRF1 qPCR of serial dilutions of pooled JY cell DNA. 1/3 indicates a 1:3 dilution of DNA from 500,000 JY cells, 1/9 indicates a 1:9 dilution of DNA from 500,000 JY cells, etcetera. B2M and BNRF1 qPCR of specific numbers of JY cells. BNRF1 was readily detectable in one single JY cell. With increasing cell numbers, the Cq value decreased correspondingly. B2M and BNRF1 qPCR of JY cells titrated into PB IgG memory B cells. D Left: B2M copies in DNA of PB IgG memory B cells and ACPA-expressing B cells as detected by qPCR. B2M was not detected in the DNA of CD40L feeder cells only. Right: no detection of BNRF1 by qPCR in DNA of PB IgG memory B cells, ACPA-expressing B cells, and CD40L feeder cells only. Each dot in IgG memory B cells represents a pool of 20 isolated and cultured cells, from seven different donors. Each dot in ACPA-expressing B cells represents a pool of 10–20 isolated and cultured cells, from seven different donors. qPCR experiments were performed twice with similar outcomes

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