Fig. 2From: Absence of Epstein-Barr virus DNA in anti-citrullinated protein antibody-expressing B cells of patients with rheumatoid arthritisBNRF1 is undetectable in PB antigen-specific ACPA-expressing B cells. A B2M and BNRF1 qPCR of serial dilutions of pooled JY cell DNA. 1/3 indicates a 1:3 dilution of DNA from 500,000 JY cells, 1/9 indicates a 1:9 dilution of DNA from 500,000 JY cells, etcetera. B B2M and BNRF1 qPCR of specific numbers of JY cells. BNRF1 was readily detectable in one single JY cell. With increasing cell numbers, the Cq value decreased correspondingly. C B2M and BNRF1 qPCR of JY cells titrated into PB IgG memory B cells. D Left: B2M copies in DNA of PB IgG memory B cells and ACPA-expressing B cells as detected by qPCR. B2M was not detected in the DNA of CD40L feeder cells only. Right: no detection of BNRF1 by qPCR in DNA of PB IgG memory B cells, ACPA-expressing B cells, and CD40L feeder cells only. Each dot in IgG memory B cells represents a pool of 20 isolated and cultured cells, from seven different donors. Each dot in ACPA-expressing B cells represents a pool of 10–20 isolated and cultured cells, from seven different donors. qPCR experiments were performed twice with similar outcomesBack to article page