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Fig. 3 | Arthritis Research & Therapy

Fig. 3

From: PSTPIP2 regulates synovial macrophages polarization and dynamics via ERβ in the joint microenvironment

Fig. 3

PSTPIP2 regulates synovial macrophages polarization in vivo. A Photographs of mouse paws and immunohistochemistry of the knee joint (brown: PSTPIP2). CIA: collagen-induced arthritis group. AAV-empty: control group. AAV-PSTPIP2: Adeno-associated virus loaded with PSTPIP2 plasmid group. B Expression of PSTPIP2 in F4/80+ synovial macrophages. F4/80+ macrophages in synovial tissue were isolated using flow sorting and then PSTPIP2 expression was detected by RT-qPCR. C Immunofluorescence double staining of synovial tissues. Green: PSTPIP2, red: F4/80. sc, synovial cavity; st, synovial tissue. D Analysis of synovial macrophages polarization: the percentage of F4/80+CD206+ cells in synovial tissue. Scatter plot on the left, histogram on the right. E Correlation analysis of PSTPIP2 level in F4/80+ synovial macrophages with the frequency of F4/80+CD206+ cells in synovial tissue. P < 0.05 indicates that the difference is statistically significant. Data represent mean ± SD (one-way ANOVA for B and D; linear regression for E)

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