Fig. 5
PSTPIP2 regulates the dynamics of synovial macrophages, which is associated with ERβ. A, B Synovial macrophages derived from the mouse synovial tissue (including AAV-empty group, AAV-PSTPIP2 group, Pstpip2CreR26-ZsGreen mice, and Esr2fl/fl mice) were fixed and stained with antibody against Actin (red) or PSTPIP2 (green). Fiji software was used to analyze the fluorescence intensity. C, F Dynamics of filopodia formation in synovial macrophages. The rate of filopodia formation in living cells was determined from time-lapse movies by counting the number of new filopodia formed in each frame. D, G Quantitative analysis of the length of filamentous protrusions. F, H Quantitative analysis of the membrane ruffling. P < 0.05 indicates that the difference is statistically significant. Data represent mean ± SD (one-way ANOVA for C–H)