Table 1 The specificity information of different monoclonal antibodies in this study
From: Rheumatoid arthritis sera antibodies to citrullinated collagen type II bind to joint cartilage
Antibodies | Origin | Classification | Isotype | Specificity |
|---|---|---|---|---|
E4 | Chimeric antibody isolated from RA patients | Promiscuous | hIgG1 | Reactive with several citrullinated peptides, including citrullinated F4 derived from COL2 [14] |
ACC4 | Triple-helical citrullinated C1 epitope immunized mice | Private specific | hIgG1 | Specific for the alpha chain of citrullinated C1 epitope on COL2 [22, 23] |
ACC1 | Triple-helical citrullinated C1 epitope immunized mice | Private cross-reactive | hIgG1 | Reactive with citrullinated C1 epitope on COL2 but also cross-reactive with several epitopes on triple-helical COL2 [22, 23] |
M2139 | COL2 immunized mice | Joint reactive | hIgG1 | Specific for the J1 epitope on triple-helical COL2 [37] |