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Fig. 4 | Arthritis Research & Therapy

Fig. 4

From: Epigenetic modification of miR-217 promotes intervertebral disc degeneration by targeting the FBXO21-ERK signalling pathway

Fig. 4

MiR-217 is involved in IDD via the FBXO21/ERK axis. A, B The Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway and gene set enrichment analyses (GSEA) identified the ERK signalling pathway as the most significantly enriched pathway in intervertebral disc degeneration. C Western blot analysis revealed that miR-217 overexpression or silencing affected the expression patterns of FBXO21, ERK, pERK, Col II, Aggrecan, MMP3, MMP13, and ADAMT5. D Western blot analysis was used to determine the expression levels of FBXO21, Col II, Aggrecan, MMP13, and ADAMT5. Overexpression of FBXO21 effectively reversed the effects of miR-217 overexpression. E Coimmunoprecipitation revealed high-affinity physical interactions between FBXO21 and ERK. IDD, intervertebral disc degeneration; miR, microRNA; Col II, type II collagen; MMP, matrix metalloprotein; ADAMTS, a disintegrin-like and metalloproteinase with thrombospondin motifs; KEGG, Kyoto Encyclopedia of Genes and Genomes; FBXO21, F-box only protein 21; ERK, extracellular regulated protein kinases

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