Fig. 1

Similar systemic autoimmune responses in B6.lpr and B6.lpr/Axl-KO mice. Mice were generated as described in the “Materials and methods” section. A Blood samples (n = 8 for each group) were collected at the age of 7, 8, 9, and 10 months. Serum anti-dsDNA levels were measured by ELISA. Antibody levels were presented as OD. B The spleens were removed from experimental animals. Single-cell splenocytes were prepared in FACS buffer and processed for surface staining. Data were acquired with the Forsseta LSR-II flow cytometer and quantified in C. Two sets of experimental mice (a total of 16 mice) were followed. Similar results were observed. Data presented are from one set. Statistical analysis was performed with an unpaired t test with Welch’s correction. ns, not significant; *p < 0.05; **p < 0.01