Fig. 2

Ameliorated lupus nephritis in B6.lpr/Axl-KO mice. B6.WT (n = 7), B6.lpr (n = 7), and B6.lpr/Axl-KO (n = 7) mice at age of 10 months were euthanized. Blood samples were collected and processed for BUN (A) measurement. Kidney sections (4 μm) were processed for H&E, trichrome, and PAS staining (B). Disease activity was quantified in C. Immune complex and complement C3 (D) staining were performed and quantified (E, F). G CD4⁺ T cells were stained, and images were taken. T cell numbers per microscopic field were counted, and averaged numbers from each mouse were presented in H. Statistical analysis was performed with an unpaired t test with Welch’s correction. ns, not significant; **p < 0.01; ***p < 0.001. Representative images were shown at × 200 magnification