Fig. 5
Reduced IL-6 levels in B6.lpr/Axl-KO mice and R428-treated nephritic B6 mice. Nephritis was induced in B6 and B6.Axl-KO mice with anti-GBM sera as previously described [47]. RNA samples were prepared from the renal cortex of experimental and B6 control mice and processed for RNA-seq analysis as described in the “Materials and methods” section. RT-PCR analysis of IL-6 expression in the cortex samples from experimental mice (as shown in Figs. 2, 3, and 4): either B6.lpr lupus mice (A) or mice treated with R428 at day 3 (B) and day 8 (C). Fold changes were then calculated using the relative CT method: fold change = 2(normalized CT from experimental mice − normalized CT.from control mice). Statistical analysis was performed with an unpaired t test with Welch’s correction. ns, not significant; *p < 0.05; **p < 0.01; ***p < 0.001